Methods Of Purification Of Organic Compounds
Chapter Name : Organic Chemistry - Some Basic Principles And Techniques |
Sub Topic Code : 102_11_12_08_04 |
Topic Name : Methods Of Purification Of Organic Compounds |
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Sub Topic Name : Differential Extraction |
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Introduction
Organic chemistry is a chemistry sub discipline involving the scientific study of the structure, properties, and reactions of organic compounds and organic materials, i.e., matter in its various forms that contain carbon atoms.
Pre-Requisites:
Organic chemistry
Activity:
Aromatic compounds.
Real Life Question:
What is organic chemistry?
Key Words / FlashCards
| Key Words | Definitions (pref. in our own words) |
|---|---|
| Differential extraction | Differential extraction refers to the process by which the DNA from two different types of cells can be extracted without mixing their contents. |
Learning aids / Gadgets
| Gadgets | How it can be used |
|---|---|
| Alcohols | Alcohols (R-OH) take the suffix "-ol" with an infix numerical bonding position: CH3CH2CH2OH is propan-1-ol. The suffixes -diol, -triol, -tetraol, etc., are used for multiple -OH groups: Ethylene glycol CH2OHCH2OH is ethane-1, 2-diol. |
Real life uses :
Study the nature of chemicals.
Places to visit :
Chemistry laboratory.
Practical examples around us
| Examples | Explainations |
|---|---|
| ketones | In general ketones (R-CO-R) take the suffix "-one" with an infix position number: CH3CH2CH2COCH3 is pentan-2-one. If a higher precedence suffix is in use, the prefix "oxo-" is used: CH3CH2CH2COCH2CHO is 3-oxohexanal. |
What you learn in Theory:
Differential extraction refers to the process by which the DNA from two different types of cells can be extracted without mixing their contents. The most common application of this method is the extraction of DNA from vaginal epithelial cells and sperm cells from sexual assault cases in order to determine the DNA profiles of the victim and the perpetrator.
What you learn in Practice:
Differential extraction uses a chemical called dithiothreitol (DTT) to disrupt the sulfur bonds in the coating of the sperm cell in order to extract its DNA.